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KMID : 1377020220190010059
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Tissue Engineering and Regenerative Medicine
2022 Volume.19 No. 1 p.59 ~ p.72
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Preparation and In Vitro Characterization of Gelatin Methacrylate for Corneal Tissue Engineering
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Yan Yayun
Cao Yanyan
Cheng Rong
Shen Zhizhong
Zhao Yajing
Zhang Yixia
Zhou Guohong
Sang Shengbo
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Abstract
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BACKGROUND: Corneal disease is second only to cataract considered as the leading cause of blindness in the world, with high morbidity. Construction of corneal substitutes in vitro by tissue engineering technology to achieve corneal regeneration has become a research hotspot in recent years. We conducted in-depth research on the biocompatibility, physicochemical and mechanical properties of rat bone marrow mesenchymal stem cells (rBM-MSCs)-seeded gelatin methacrylate (GelMA) as a bioengineered cornea.
METHODS: Four kinds of GelMA with different concentrations (7, 10, 15 and 30%) were prepared, and their physic-chemical, optical properties, and biocompatibility with rBM-MSCs were characterized. MTT, live/dead staining, cell morphology, immunofluorescence staining and gene expression of keratocyte markers were performed.
RESULTS: 7%GelMA hydrogel had higher equilibrium water content and porosity, better optical properties and hydrophilicity. In addition, it is more beneficial to the growth and proliferation of rBM-MSCs. However, the 30%GelMA hydrogel had the best mechanical properties, and could be more conducive to promote the differentiation of rBM-MSCs into keratocyte-like cells.
CONCLUSION: As a natural biological scaffold, GelMA hydrogel has good biocompatibility. And it has the ability to promote the differentiation of rBM-MSCs into keratocyte-like cells, which laid a theoretical and experimental foundation for further tissue-engineered corneal stromal transplantation, and provided a new idea for the source of seeded cells in corneal tissue engineering.
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KEYWORD
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Bioengineered cornea, Bone marrow mesenchymal stem cells, Corneal tissue engineering, Gelatin methacrylate, Hydrogel
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